Anti-OTUB1 Polyclonal AntibodyAnti--上海极威生物商城-Elisa试剂盒,白介素ELISA试剂盒,酶联免疫试剂盒,Gibco胎牛血清,Hyclone胎牛血清,胎牛血清等实验耗材一站式采购平台

产品简介

英文名称	Anti-OTUB1 Polyclonal Antibody
别名	HSPC263;OTB1;OTU1
宿主	Rabbit
稀释比例	WB 1:1000-3000.
Gene ID	55611
保存	Store at -20°C. Avoid freeze / thaw cycles.
Swiss Prot	Q96FW1
蛋白分子量	31/35kDa
储存液	Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
亚细胞定位	Cytoplasm
免疫原	Recombinant protein of human OTUB1
亚型	IgG
纯化方法	Affinity purification
背景资料	Hydrolase that can specifically remove 'Lys-48'-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin.Plays a key non-catalytic role in DNA repair regulation by inhibiting activity of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites. Inhibits RNF168 independently of ubiquitin thioesterase activity by binding and inhibiting UBE2N/UBC13, the E2 partner of RNF168, thereby limiting spreading of 'Lys-63'-linked histone H2A and H2AX marks. Inhibition occurs by binding to free ubiquitin: free ubiquitin acts as an allosteric regulator that increases affinity for UBE2N/UBC13 and disrupts interaction with UBE2V1. The OTUB1-UBE2N/UBC13-free ubiquitin complex adopts a configuration that mimics a cleaved 'Lys48'-linked di-ubiquitin chain.
Public Immunogen Range	1-271aa
克隆类型	Polyclonal Antibody
交叉反应	HumanRatMouse
应用	WB
规格	50ul100ul