| 英文名称 | Anti-Phospho-IKK alpha/beta (Ser176 + Ser180) Polyclonal Antibody |
| 别名 | IKK alpha (phospho S176 + S180);p-IKK alpha (phospho S176 + S180);I Kappa B Kinase Alpha;IKKalpha;IKK alpha;IkappaB kinase;IkB kinase alpha subunit;IKBKA;IKK 1;IKK A;IKK a kinase;IKK1;IKKA;Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells;Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit;NFKBIKA;Nuclear Factor Kappa B Inhibitor Kinase Alpha |
| 宿主 | Rabbit |
| 稀释比例 | ELISA 1:500-1000. IHC-P 1:100-500. IHC-F 1:100-500. IF 1:100-500. |
| Gene ID | 1147 |
| 保存 | Store at -20°C. Avoid freeze / thaw cycles. |
| Swiss Prot | O15111 |
| 蛋白分子量 | 85/87kDa |
| 储存液 | Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3. |
| 亚细胞定位 | Cytoplasm Nucleus |
| 免疫原 | KLH conjugated synthesised phosphopeptide derived from human IKK alpha/beta around the phosphorylation site of Ser176/Ser180 |
| 亚型 | IgG |
| 纯化方法 | Affinity purification |
| 背景资料 | Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3. |
| 克隆类型 | Polyclonal Antibody |
| 交叉反应 | HumanDogpigCowHorseChickenRatMouse |
| 应用 | ELISAIFIHC-FIHC-P |
| 规格 | 50ul100ul |
