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ELISA试剂盒

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高敏ELISA试剂盒

高敏人Elisa试剂盒| 高敏小鼠Elisa试剂盒| 高敏大鼠Elisa试剂盒| 高敏豚鼠Elisa试剂盒| 高敏裸鼠Elisa试剂盒| 高敏仓鼠Elisa试剂盒| 高敏沙鼠Elisa试剂盒| 高敏鸭Elisa试剂盒| 高敏鹅Elisa试剂盒| 高敏猴Elisa试剂盒| 高敏兔Elisa试剂盒| 高敏马Elisa试剂盒| 高敏绵羊Elisa试剂盒| 高敏山羊Elisa试剂盒| 高敏犬Elisa试剂盒| 高敏牛Elisa试剂盒| 高敏鱼Elisa试剂盒|

技术服务

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染色试剂&糖类

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荧光定量比色法试剂盒

细胞技术类产品| 生化试剂盒| 分子技术类产品| 蛋白化学技术类产品| 免疫抗体技术类产品| 医学技术类产品| 病理技术类产品| 生物化学技术类产品| 模式生物技术类产品| 微生物技术类产品| 植物技术类产品| 载体技术类产品| 毒理技术类产品| 营养技术类产品| 平台技术类产品| 其他相关产品|

血清抗体纯化试剂盒

蛋白纯化试剂盒| 抗体| 血清|

生化试剂

蛋白质类| 氨基酸&多肽&蛋白质| 抗生素(生化试剂)| 酶&辅酶&抑制剂| 动植物激素| 碳水化合物及衍生物| 色素类| 维生素| 分离材料及耗材| 表面活性剂| 缓冲溶剂| 其他化学试剂| 碱基&核酸及其衍生物| 酸&盐&胺| 常规生化试剂|

细胞生物学

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抗体

正常动物血清及免疫球蛋白| 标记蛋白质与多肽| 蛋白质与多肽| 标记二抗| 二抗| 标记一抗| 一抗| 内参抗体| 标签抗体| 抗原| 植物抗体|

培养基

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分析对照品、标准品

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仪器耗材

酶标仪| 常规耗材| 进口耗材| 移液器| 其他小仪器|

进口试剂

Life试剂| Amresco试剂| Sigma试剂| R&D试剂| Merck试剂| Novus试剂| Lifespan试剂| eBioscience| Pepro Tech| Gene Tex| Cayman| ENZO| Serotec| Active Motif| InvivoGen| ProZyme| Vetorlabs| Mirus| Fitzgerald| Biovendor| BioVision|

生化试剂盒

其它系列| ELISA试剂盒| 放免试剂盒| 辅酶Ⅰ系列| 辅酶Ⅱ系列| 谷胱甘肽系列| 维生素C代谢系列| 氧化与抗氧化系列| 氮代谢系列| 氨基酸代谢系列| 微量法| 常量法| 高效液相色谱法| 抗逆系列| 氧化系列| 抗氧化系列| 糖酵解系列| ATP系列| 酯酶系列| 线粒体呼吸链系列| 三羧酸循环系列| 蛋白酶系列| 脂肪酸代谢系列| 淀粉系列| 蔗糖系列| 糖代谢系列| 离子系列| 土壤系列| 信号系列| 转氢酶系列| 蛋白定量| 果胶系列| 光合作用系列| 糖异生系列| 维生素系列|

首页 > 血清抗体纯化试剂盒 > 蛋白纯化试剂盒
抗体小量纯化试剂盒
产品名称:
抗体小量纯化试剂盒
英文名称:
Antibody Purification Mini Kit
型号:
GV-CY-18110
产品库存
100
产品价格
电议

产品详情

抗体小量纯化试剂盒

编号 GV-CY-18110

类型 蛋白纯化

规格 10T

材质 lmmobilized Protein A

颜色 液体

品牌 极威生物

型号 小量纯化

产品详情

 

填料为原装的GE!!国产的价格!!

抗体小量纯化试剂盒,来源于金黄色葡萄球菌的蛋白A与抗体的不变区有多个结合位点。将蛋白A与Sepharose共价交联制备的层析介质,可以用于抗体纯化。抗体与蛋白A在高盐高pH值条件下结合,在低盐pH值条件下解离。不同来源的抗体与蛋白A的结合能力有较大差异,结合与解离的条件也有区别。

 

订货信息

中文名称:抗体小量纯化试剂盒

英文名称:Antibody Purification Mini Kit

品      牌:极威生物

产品货号:GV-CY-18110

规      格:10T

 

价      格:咨询客服

 

Protein A对不同来源IgG结合剂量 

 

高亲和性(如兔)

低亲和性(如小鼠)

Protein A Sepharose 4 FF结合剂量

35 mg/ml

3-10 mg/ml

Protein A Sepharose 4 FF每次用量

40微升

40微升(50%悬液)

每次纯化抗体数量

700微克

60-200微克

 

一般高滴度抗血清中IgG浓度

 

小鼠

兔抗血清

IgG浓度

10 mg/ml

5 mg/ml

20 mg/ml

 

Mini kit               

PRE-EQUILIBRATION

1.Equilibrate the Spin Column with0.6mL Binding Buffer A by centrifuging the spin column at 500 × g for1minutes.

NOTE: If using one spin column, ensure that the spin column is counterbalanced with a unit of equal weight (adjusted

with distilled water).

CLARIFICATION OF SAMPLE

2.Pre-filter the sample (e.g., tissue culture supernatant, serum or ascites) through a 0.22 μmSyringeFilter toremoveany debris immediately before loading the sample.Protein precipitation is common during storage and when repeatedfreeze/thaw cycles in ascites, sera and tissue culture supernatants occur. Newly formed aggregates and precipitatingprotein complexes can foul theProteinA media and result in significantly slower flow rates. Ensure that thesamples are filteredimmediatelybefore loading, using filters with pore sizes no greater than 0.22 μm. It is critical tothe optimal performance of these devices that these instructions are rigorously followed.

SAMPLE LOADING

3.Dilute the filtered sample 1:1 v/v in Binding Buffer A. (For example, add 1 mL filtered sample to 1 mL buffer.) Pipettethe0.6mL sample into the spin column. Centrifuge the spin column at 100–150 × g for1minutes.Ideal

sample loadingconditions are obtained using a flow rate of less than 1 mL/min. It may be necessary to increase the spin time or spinspeed if any sample remainsincolumn. Alternatively, a flow rate slower than expected is indicative

of a partiallyclogged plug resulting from incomplete filtration of the sample.

WASHING

4.Wash the spin column to remove unbound contaminants by adding0.6mL Binding Buffer A and centrifuging the spincolumn for1minutes at 500 × g. Add another0.6mL Binding Buffer A and centrifuge for2more minutes at 500 × g.Theunbound wash will contain non-immunoglobulin components. A flow rate slower than expected is indicative of apartially clogged plug resulting from incomplete filtration of the sample. In the unlikely event that flow rates are

significantly slower than those expected, increase the centrifugal speed to 1000 × g with2minute spin time bursts.

ELUTION

■For purifying mouse IgG1, rat IgG1, rat IgG2a, rat IgG2b and bovine IgG1 (or if you are unsure which IgG subclass youare purifying), use both elution steps 5 and 6 for your initial kit use in order to establish the mildest elution conditionpossible for the antibody. Analyze the two fractions in separate tubes to avoid sample dilution.

■For purifying mouse IgG2a, mouse IgG2b, mouse IgG3, rat IgG2c, human IgG1-IgG4, rabbit IgG, guinea pig IgG1,

guinea pig IgG2, bovine IgG2 and any other IgGs, proceed to elution step 6 only.

■A flow rate slower than expected is indicative of a partially clogged plug resulting from incomplete filtration of the

sample. In the unlikely event that flow rates are significantly slower than those expected, increase the centrifugal speedto 1000 × g with2minute spin time bursts.

5.Elute the bound IgG with0.1mL Elution Buffer B1 directly into a fresh centrifuge tube containing 0.005 mL NeutralizationBuffer C to bring the sample to neutral pH. Centrifuge the spin column for 5 minutes at 500 × g.Save the sample for analysis

6.Elute the bound IgG with0.1mL Elution Buffer B2 directly into a fresh centrifuge tube containing0.013 mL NeutralizationBuffer C to bring the sample to neutral pH. Centrifuge the spin column for 5 minutes at 500 × g.Save the sample for analysis.

REGENERATION

7.Wash the media with0.6mL Elution Buffer B2 by centrifuging the spin column at 500 × g for2minutes.

Re-equilibrate themedia with0.6mL of Binding Buffer A by centrifuging the spin column at 500 × g for 2 minutes.

DESALTING AND CONCENTRATING

8.If necessary, desalt and concentrate the antibody preparation using the Ultra centrifugal filter device with30,000 NMWL. Add 0.05-0.5% w/v sodium azide if the antibodies are to be stored at 2–8 °C.

We recommend freezing the antibodies in small aliquots in 50% glycerol at -20 °C for long term storage.

 

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