CAT#:JW-1-2370
低温运输，-20℃保存

产品及特点
本产品为pCold TF质粒DNA，是一种以大肠杆菌的伴侣蛋白Trigger Factor(TF)作为可溶性标签的融合型冷休克表达载体，具有CSPA启动子。pCold TF质粒DNA具有多克隆位点，方便用户在不同酶切位点插入所选基因进行研究或表达。本质粒携带Amp抗性基因，其图谱如下：

规格及成分
本产品使用塑料袋包装
成分                                    编号    规格    包装
pCold TF质粒DNA干粉    1-2370    2 mg    0.5 mL本色管
使用手册                         1-2370sc    1份    无

运输及保存
低温运输，-20℃保存，有效期两年

使用方法
使用前将本产品短暂离心后加入100μL自备超纯水或TE缓冲液溶解，得到20ng/μL的质粒DNA溶液，该溶液可直接用于酶切克隆。也可以取少量，按常规方法进行细菌转化，制备更多的质粒DNA进行各种后续实验。

DNA序列
11 aaggaatggt gtggccgatt aatcataaat atgaaaaata attgttgcat 50
151 cacccgccaa tgcgtggctt aatgcacatc aaattgtgag cggataacaa 100
101 tttgatgtgc tagcgcatat ccagtgtagt aaggcaagtc ccttcaagag 150
151 ttatcgttga tacccctcgt agtgcacatt cctttaacgc ttcaaaatct 200
201 gtaaagcacg ccatatcgcc gaaaggcaca cttaattatt aagaggtaat 250
251 acaccatgaa tcacaaagtg catcatcatc atcatcacat gcaagtttca 300
301 gttgaaacca ctcaaggcct tggccgccgt gtaacgatta ctatcgctgc 350
351 tgacagcatc gagaccgctg ttaaaagcga gctggtcaac gttgcgaaaa 400
401 aagtacgtat tgacggcttc cgcaagggca aagtgccaat gaatatcgtt 450
451 gctcagcgtt atggcgcgtc tgtacgccag gacgttctgg gtgacctgat 500
501 gagccgtaac ttcattgacg ccatcattaa agaaaaaatc aatccggctg 550
551 gcgcaccgac ttatgttccg ggcgaataca agctgggtga agacttcact 600
601 tactctgtag agtttgaagt ttatccggaa gttgaactgc aaggtctgga 650
651 agcgatcgaa gttgaaaaac cgatcgttga agtgaccgac gctgacgttg 700
701 acggcatgct ggatactctg cgtaaacagc aggcgacctg gaaagaaaaa 750
751 gacggcgctg ttgaagcaga agaccgcgtg accatcgact tcaccggttc 800
801 tgtagacggc gaagagttcg aaggcggtaa agcgtctgat ttcgtactgg 850
851 cgatgggcca gggtcgtatg atcccgggct ttgaagacgg tatcaaaggc 900
901 cacaaagctg gcgaagagtt caccatcgac gtgaccttcc cggaagaata 950
1951 ccacgcagaa aacctgaaag gtaaagcagc gaaattcgct atcaacctga 1000
1001 agaaagttga agagcgtgaa ctgccggaac tgaccgcaga gttcatcaaa 1050
1051 cgtttcggcg ttgaagatgg ttccgtagaa ggtctgcgcg ctgaagtgcg 1100
1101 taaaaacatg gagcgcgagc tgaagagcgc catccgtaac cgcgttaagt 1150
1151 ctcaggcgat cgaaggtctg gtaaaagcta acgacatcga cgtaccggct 1200
1201 gcgctgatcg acagcgaaat cgacgttctg cgtcgccagg ctgcacagcg 1250
1251 tttcggtggc aacgaaaaac aagctctgga actgccgcgc gaactgttcg 1300
1301 aagaacaggc taaacgccgc gtagttgttg gcctgctgct gggcgaagtt 1350
1351 atccgcacca acgagctgaa agctgacgaa gagcgcgtga aaggcctgat 1400
1401 cgaagagatg gcttctgcgt acgaagatcc gaaagaagtt atcgagttct 1450
1451 acagcaaaaa caaagaactg atggacaaca tgcgcaatgt tgctctggaa 1500
1501 gaacaggctg ttgaagctgt actggcgaaa gcgaaagtga ctgaaaaaga 1550
1551 aaccactttc aacgagctga tgaaccagca ggcgtccgcg ggtctggaag 1600
1601 ttctgttcca ggggccctcc gcgggtctgg tgccacgcgg tagtggtggt 1650
1651 atcgaaggta ggcatatgga gctcggtacc ctcgagggat ccgaattcaa 1700
1701 gcttgtcgac ctgcagtcta gataggtaat ctctgcttaa aagcacagaa 1750
1751 tctaagatcc ctgccatttg gcggggattt ttttatttgt tttcaggaaa 1800
1801 taaataatcg atcgcgtaat aaaatctatt attatttttg tgaagaataa 1850
1851 atttgggtgc aatgagaatg cgcaggccct ttcgtctcgc gcgtttcggt 1900
1901 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 1950
1951 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 2000
2001 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag 2050
2051 attgtactga gagtgcacca taaaattgta aacgttaata ttttgttaaa 2100
2101 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 2150
2151 aaatcggcaa aatcccttat aaatcaaaag aatagcccga gatagggttg 2200
2201 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 2250
2251 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg 2300
2301 aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta 2350
2351 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc 2400
2401 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 2450
2451 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc 2500
2501 gcgcttaatg cgccgctaca gggcgcgtac tatggttgct ttgacgtatg 2550
2551 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 2600
2601 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 2650
2651 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 2700
2701 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 2750
2751 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc 2800
2801 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 2850
2851 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 2900
2901 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 2950
2951 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 3000
3001 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 3050
3051 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc 3100
3101 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 3150
3151 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 3200
3201 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 3250
3251 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 3300
3301 aactattaac tggcgaacta cttactctag cttcccggca acaattaata 3350
3351 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct 3400
3401 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 3450
3451 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 3500
3501 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 3550
3551 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 3600
3601 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 3650
3651 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 3700
3701 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 3750
3751 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 3800
3801 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 3850
3851 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 3900
3901 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac 3950
3951 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 4000
4001 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 4050
4051 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 4100
4101 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 4150
4151 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 4200
4201 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 4250
4251 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 4300
4301 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 4350
4351 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 4400
4401 tttgctcaca tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt 4450
4451 gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct 4500
4501 aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 4550
4551 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 4600
4601 tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc 4650
4651 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg 4700
4701 gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta 4750
4751 acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactacc 4800
4801 gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc 4850
4851 gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc 4900
4901 cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag 4950
4951 tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt 5000
5001 atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg 5050
5051 ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc 5100
5101 agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg 5150
5151 gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca 5200
5201 cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg 5250
5251 acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc 5300
5301 gcttcgttct accatcgaca ccaccacgct ggcacccagt tgatcggcgc 5350
5351 gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg 5400
5401 gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc 5450
5451 cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt 5500
5501 cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg 5550
5551 gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg 5600
5601 tttcacattc accaccctga attgactctc ttccgggcgc tatcatgcca 5650
5651 taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc 5700
5701 tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc 5750
5751 cgttgagcac cgccgccgc 5769 ttaggaagca gcccagtagt aggttgaggcC

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