CAT#:JW-1-1440
常温运输及保存

产品及特点
本产品为pcDNA3.1(+)质粒DNA干粉。pcDNA3.1(+)质粒DNA是含有CMV、T7、SV40及lac启动子的哺乳细胞表达载体，具有多克隆位点，方便用户在不同酶切位点插入所选基因进行研究或表达。本质粒携带卡那霉素/新霉素抗性基因和氨苄青霉素抗性基因。其图谱如下：

规格及成分
本产品使用塑料袋包装
成分                                        编号            规格    包装
pcDNA3.1(+)质粒DNA干粉    1-1440        2 mg    0.5 mL本色管
使用手册                                1-1440sc    1 份    无

运输及保存
常温运输及保存，有效期两年。

使用方法
使用前将本产品短暂离心后加入100μL自备超纯水或TE缓冲液溶解，得到20ng/μL的质粒DNA溶液，该溶液可直接用于酶切克隆。也可以取少量，按常规方法进行细菌转化，制备更多的质粒DNA进行各种后续实验。注意：加水溶解后的质粒DNA溶液需-20℃保存。
DNA序列

1 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
151 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
101 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
151 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
201 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
251 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
351 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
401 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
451 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
501 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
551 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
651 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
701 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
751 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
801 gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
851 gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt
951 ggaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc
1001 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
1051 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
1101 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
1151 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
1201 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
1251 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
1301 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
1351 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
1401 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
1451 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
1501 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
1551 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
1601 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
1651 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
1701 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
1751 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
1801 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
1851 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
1901 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
1951 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
2001 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
2051 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
2101 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
2151 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
2201 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
2251 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
2301 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
2351 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
2401 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
2451 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
2501 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
2551 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
2601 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
2651 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
2701 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
2751 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
2801 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
2851 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
2901 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
2951 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
3001 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
3051 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
3101 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
3151 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
3201 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
3251 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
3301 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
3351 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
3401 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
3451 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
3501 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
3551 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
3601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
3651 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
3701 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
3751 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
3801 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
3851 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
3901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
3951 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
4001 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
4051 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
4101 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
4151 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
4201 cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
4251 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
4301 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
4351 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
4401 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
4451 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
4501 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
4551 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
4601 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
4651 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
4701 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
4751 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
4801 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
4851 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
4901 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
4951 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
5001 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
5051 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
5101 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
5151 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
5201 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
5251 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
5301 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
5351 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
5401 atttccccga aaagtgccac ctgacgtcac tggatctcaa cagcggtaag

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