CAT#:JW-1-1220
常温运输及保存

产品及特点
本产品为pCAMBIA1300质粒DNA，是具有Lac启动子的农杆菌双元表达载体。pCAMBIA1300质粒DNA具有多克隆位点，方便用户在不同酶切位点插入所选基因进行研究或表达。本质粒携带卡那霉素及潮霉素抗性基因，其图谱如下：

规格及成分
本产品使用塑料袋包装
成分    编号    规格    包装
pCAMBIA1300质粒DNA干粉    1-1220    2 mg    0.5 mL本色管
使用手册    1-1220sc    1份    无

运输及保存
常温运输及保存，有效期两年

自备物品
超纯水

使用方法
使用前将本产品短暂离心后加入100μL自备超纯水或TE缓冲液溶解，得到20ng/μL的质粒DNA溶液，该溶液可直接用于酶切克隆。也可以取少量，按常规方法进行细菌转化，制备更多的质粒DNA进行各种后续实验。

DNA序列
1 11 aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc
 151 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
 101 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
 151 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
 201 cctgaatggc gaatgctaga gcagcttgag cttggatcag attgtcgttt
 251 cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta
 301 aacctaagag aaaagagcgt ttattagaat aacggatatt taaaagggcg
 351 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
 401 ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg
 451 cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg
 501 cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg
 551 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga
 601 accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc
 651 tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga
 701 actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca
 751 ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct
 801 ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg
 851 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc
 901 gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg
 951 gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat
1001 cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt
1051 ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag
1101 ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg
1151 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga
1201 cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc
1251 gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc
1301 atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga
1351 gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc
1401 acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc
1451 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg
1501 ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg
1551 tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac
1601 gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg
1651 accatcgcaa cccatctagc ccgcgccctG caactcgccg gggccgatgt
1701 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg
1751 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt
1801 gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg
1851 agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact
1901 tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc
1951 gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct
2001 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg
2051 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc
2101 cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac
2151 cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc
2201 tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga
2251 aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc
2301 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa
2351 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt
2401 acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc
2451 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt
2501 tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
2551 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag
2601 cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg
2651 aggaatcggc gtgaGcggtc gcaaaccatc cggcccggta caaatcggcg
2701 cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc
2751 cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc
2801 ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
2851 cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc
2901 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga
2951 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga
3001 tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc
3051 gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat
3101 gctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
3151 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga
3201 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt
3251 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc
3301 gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc
3351 gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga
3401 ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg
3451 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac
3501 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa
3551 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt
3601 tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg
3651 aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa
3701 cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc
3751 tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct
3801 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa
3851 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt
3901 aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct
3951 ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc
4001 tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc
4051 tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc
4101 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca
4151 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc
4201 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
4251 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
4301 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
4351 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
4401 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
4451 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
4501 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
4551 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
4601 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
4651 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
4701 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
4751 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
4801 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
4851 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
4901 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
4951 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
5001 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
5051 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
5101 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
5151 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
5201 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
5251 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
5301 aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc
5351 atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca
5401 gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg
5451 atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
5501 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt
5551 tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt
5601 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc
5651 ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt
5701 aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc
5751 gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
5801 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc
5851 catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag
5901 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc
5951 cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca
6001 tttttaaata taggttttca ttttctccca ccagcttata taccttagca
6051 ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
6101 tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc
6151 ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
6201 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca
6251 gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga
6301 gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt
6351 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
6401 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc
6451 cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc
6501 tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc
6551 tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa
6601 cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt
6651 aattcggggg atctggattt tagtactgga ttttggtttt aggaattaga
6701 aattttattg atagaagtat tttacaaata caaatacata ctaagggttt
6751 cttatatgct caacacatga gcgaaaccct ataggaaccc taattccctt
6801 atctgggaac tactcacaca ttattatgga gaaactcgag cttgtcgatc
6851 gacagatccg gtcggcatct actctatttc tttgccctcg gacgagtgct
6901 ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
6951 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct
7001 ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc
7051 gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga
7101 gcatatacgc ccggagtcgt ggcgatcctg caagctccgg atgcctccgc
7151 tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa
7201 gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
7251 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag
7301 ccgaaatccg cgtgcacgag gtgccggact tcggggcagt cctcggccca
7351 aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt
7401 ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg
7451 aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc
7501 gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatagcct
7551 ccgcgaccgg ttgtagaaca gcgggcagtt cggtttcagg caggtcttgc
7601 aacgtgacac cctgtgcacg gcgggagatg caataggtca ggctctcgct
7651 aaactcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa
7701 agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt
7751 acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga
7801 ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
7851 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata
7901 tctcattgcc ccccgggatc tgcgaaagct cgagagagat agatttgtag
7951 agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct
8001 tatatagagg aaggtcttgc gaaggatagt gggattgtgc gtcatccctt
8051 acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg
8101 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
8151 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa
8201 tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa
8251 gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc
8301 tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg
8351 atattcttgg agtagacgag agtgtcgtgc tccaccatgt tatcacatca
8401 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
8451 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct
8501 tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
8551 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga
8601 atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
8651 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
8701 gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgcc
8751 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
8801 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
8851 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
8901 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
8951 atgattacgg catcatcagg agtacggata aaatgcttga tggtcggaag

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