CAT#:JW-1-286
常温运输，低温保存

产品及特点
本产品为pAc5.1/V5-His A质粒DNA，是具有Ac5启动子的昆虫表达载体。pAc5.1/V5-His A质粒DNA具有多克隆位点，方便用户在不同酶切位点插入所选基因进行研究或表达。pAc5.1/V5-His A质粒DNA多克隆位点下游带有V5标签和6X His标签编码区，使得表达的蛋白的C端带V5标签和6X His标签，便于后续用V5抗体和镍柱进行蛋白纯化。本质粒携带Amp抗性基因，其图谱如下：

规格及成分
本产品使用塑料袋包装
成分                                                编号    规格    包装
pAc5.1/V5-His A质粒DNA干粉    1-286    2 μg    0.5 mL本色管
使用手册                                    1-286sc    1份    无

运输及保存
常温运输，低温保存，有效期两年。

使用方法
使用前将本产品短暂离心后加入100μL自备超纯水或TE缓冲液溶解，得到20ng/μL的质粒DNA溶液，该溶液可直接用于酶切克隆。也可以取少量，按常规方法进行细菌转化，制备更多的质粒DNA进行各种后续实验。
DNA序列
1 11 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa
 151 aaaatcatga atggcatcaa ctctgaatca aatctttgca gatgcaccta
 101 cttctcattt ccactgtcac atcatttttc cagatctcgc tgcctgttat
 151 gtggcccaca aaccaagaca cgttttatgg ccattaaagc tggctgatcg
 201 tcgccaaaca ccaaatacat atcaatatgt acattcgaga aagaagcgat
 251 caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
 301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca
 351 tattcggagc aatttgaagc caatttccat cgcctggcga tcgctccatt
 401 cttggctata tgtttttcac cgttcccggg gccattttca aagactcgtc
 451 ggtaagataa gattgtgtca ctcgctgtct ctcttcattt gtcgaagaat
 501 gctgaggaat ttcgcgatga cgtcggcgag tattttgaag aatgagaata
 551 atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
 601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt
 651 atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca
 701 atgatgatac tgatgatatt ttaagatgat gccagaccac aggctgattt
 751 ctgcgtcttt tgccgaacgc agtgcatgtg cggttgttgt tttttggaat
 801 agtttcaatt ttcggactgt ccgctttgat ttcagtttct tggcttattc
 851 aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
 901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg
 951 cggggcgtgg cggggtctgt ggctgggcgc gacgtcaccg acgttggagc
1001 cactcctttg accatgtgtg cgtgtgtgta ttattcgtgt ctcgccactc
1051 gccggttgtt tttttctttt tatctcgctc tctctagcgc catctcgtac
1101 gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg tcattttggc
1151 tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg
1251 atagcaacaa aagcaacaac acagtggctt tccctctttc actttttgtt
1301 tgcaagcgcg tgcgagcaag acggcacgac cggcaaacgc aattacgctg
1351 acaaagagca gacgaagttt tggccgaaaa acatcaaggc gcctgatacg
1401 aatgcatttg caataacaat tgcgatattt aatattgttt atgaagctgt
1451 ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg
1551 aaatagctta cctgacgtca cagcctctgg aagaactgcc gccaagcaga
1601 gagagagaga aaaagaggga gagcagctta gaccgcatgt gcttgtgtgt
1651 gaggcgtctc tctcttcgtc tcctgtttgc gcaaacgcat agactgcact
1701 gagaaaatcg attacctatt ttttatgaat gaatatttgc actattacta
1751 ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt
1851 aggacggcat cattatagaa atgcttcttg ctgtgtactt ttctctcgtc
1901 tggcagctgt ttcgccgtta ttgttaaaac cggcttaagt taggtgtgtt
1951 ttctacgact agtgatgccc ctactagaag atgtgtgttg cacaaatgtc
2001 cctgaataac caatttgaag tgcagatagc agtaaacgta agctaatatg
2051 aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt
2151 ggggaaaaaa tgtgtaaaag caccgtgacc atcacagcat aaagataacc
2201 agctgaagta tcgaatatga gtaaccccca aattgaatca catgccgcaa
2251 ctgataggac ccatggaagt acactcttca tggcgatata caagacacac
2301 acaagcacga acacccagtt gcggaggaaa ttctccgtaa atgaaaaccc
2351 aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt
2451 tataaaacgg gctgcgggac cagttttcat atcactaccg tttgagttct
2501 tgtgctgtgt ggatactcct cccgacacaa agccgctcca tcagccagca
2551 gtcgtctaat ccagagaccc cggatcgggg tacctactag tccagtgtgg
2601 tggaattctg cagatatcca gcacagtggc ggccgctcga gtctagaggg
2651 cccttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
2701 gcgtaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag
2751 cctcgactgt gccttctaag gcctgagctc gctgatcagc ctcgatcgag
2801 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
2851 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
2901 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
2951 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
3001 caagtaaaac ctctacaaat gtggtatggc tgattatgat cagtcgaccg
3051 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
3101 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
3151 taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt
3201 cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt
3251 gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg
3301 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac
3351 gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat
3401 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt
3451 ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg
3501 gctcttacca gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
3551 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
3601 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
3651 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
3701 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
3751 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
3801 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
3851 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
3901 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
3951 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
4001 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
4051 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
4101 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
4151 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
4201 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
4251 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
4301 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
4351 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
4401 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
4451 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
4501 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
4551 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
4601 taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
4651 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
4701 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
4751 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
4801 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
4851 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
4901 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
4951 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
5001 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
5051 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
5101 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
5151 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
5201 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
5251 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
5301 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
5351 taggcgtatc acgaggccct ttcgtttatc atgacattaa cctataaaaa

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