CAT#:1-282
低温运输，-20℃保存


产品及特点
本产品为pAAV-IRES-hrGFP质粒DNA溶液。pAAV-IRES-hrGFP质粒DNA是具有CMV启动子、pUC复制子和PolyA转录终止信号的腺病毒包装载体。pAAV-IRES-hrGFP质粒DNA携带hrGFP基因（人源化绿色荧光蛋白基因，Humanized Renilla reniformis Green Fluorescent Protein Gene），用户可根据实验需求将外源DNA插入hrGFP结构基因上游进行共表达实验。本质粒携带Amp抗性基因。其图谱如下：

规格及成分
本产品使用塑料袋包装
成分                                    编号       规格    包装
pAAV-IRES-hrGFP质粒
DNA溶液，100 ng/μL       1-282        20 μL    0.5 mL本色管
使用手册                            1-282sc    1 份    无

运输及保存
低温运输，-20℃保存，有效期两年。

使用方法
pAAV-IRES-hrGFP质粒DNA溶液可以直接用于腺病毒包装实验。也可以取少量，按常规方法进行细菌转化，制备更多的质粒DNA进行各种后续实验。

DNA序列
11 1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG
 151 CCCGGGCGTC GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC
 101 GCGCAGAGAG GGAGTGGCCA ACTCCATCAC TAGGGGTTCC TGCGGCCGCA
 151 CGCGTGGAGC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT
 201 AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC
 251 TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG
 301 TTCCCATAGT AACGTCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG
 351 TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC
 401 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT
 451 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC
 501 GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA
 551 TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA
 601 TTGACGTCAAT GGGAGTTTGT TTTGCACCA AAATCAACGG GACTTTCCAA
 651 AATGTCGTAA CAACTCCGCC CCATTGACGC AAATGGGCGG TAGGCGTGTA
 701 CGGTGGGAGG TCTATATAAG CAGAGCTCGT TTAGTGAACC GTCAGATCGC
 751 CTGGAGACGC CATCCACGCT GTTTTGACCT CCATAGAAGA CACCGGGACC
 801 GATCCAGCCT CCGCGGATTC GAATCCCGGC CGGGAACGGT GCATTGGAAC
 851 GCGGATTCCC CGTGCCAAGA GTGACGTAAG TACCGCCTAT AGAGTCTATA
 901 GGCCCACAAA AAATGCTTTC TTCTTTTAAT ATACTTTTTT GTTTATCTTA
 951 TTTCTAATAC TTTCCCTAAT CTCTTTCTTT CAGGGCAATA ATGATACAAT
1001 GTATCATGCC TCTTTGCACC ATTCTAAAGA ATAACAGTGA TAATTTCTGG
1051 GTTAAGGCAA TAGCAATATT TCTGCATATA AATATTTCTG CATATAAATT
1101 GTAACTGATG TAAGAGGTTT CATATTGCTA ATAGCAGCTA CAATCCAGCT
1151 ACCATTCTGC TTTTATTTTA TGGTTGGGAT AAGGCTGGAT TATTCTGAGT
1201 CCAAGCTAGG CCCTTTTGCT AATCATGTTC ATACCTCTTA TCTTCCTCCC
1251 ACAGCTCCTG GGCAACGTGC TGGTCTGTGT GCTGGCCCAT CACTTTGGCA
1301 AAGAATTGGG ATTCGAACAT CGATAATTAA CCCTCACTAA AGGGAACAAA
1351 AGCTGGAGCT CCACCGCGGT GGCGGCCGCT CTAGCCCGGG CGGATCCGAA
1401 TTCGCATGCG TCGACTCGAG GACTACAAGG ATGACGATGA CAAGGATTAC
1451 AAAGACGACG ATGATAAGGA CTATAAGGAT GATGACGACA AATAATAGCA
1501 ATTCCTCGAC GACTGCATAG GGTTACCCCC CTCTCCCTCC CCCCCCCCTA
1551 ACGTTACTGG CCGAAGCCGC TTGGAATAAG GCCGGTGTGC GTTTGTCTAT
1601 ATGTTATTTT CCACCATATT GCCGTCTTTT GGCAATGTGA GGGCCCGGAA
1651 ACCTGGCCCT GTCTTCTTGAC GAGCATTCC TAGGGGTCTT TCCCCTCTCG
1701 CCAAAGGAAT GCAAGGTCTG TTGAATGTCG TGAAGGAAGC AGTTCCTCTG
1751 GAAGCTTCTT GAAGACAAAC AACGTCTGTA GCGACCCTTT GCAGGCAGCG
1801 GAACCCCCCA CCTGGCGACA GGTGCCTCTG CGGCCAAAAG CCACGTGTAT
1851 AAGATACACC TGCAAAGGCG GCACAACCCC AGTGCCACGT TGTGAGTTGG
1901 ATAGTTGTGG AAAGAGTCAA ATGGCTCTCC TCAAGCGTAT TCAACAAGGG
1951 GCTGAAGGAT GCCCAGAAGG TACCCCATTG TATGGGATCT GATCTGGGGC
2001 CTCGGTGCAC ATGCTTTACA TGTGTTTAGT CGAGGTTAAA AAACGTCTAG
2051 GCCCCCCGAA CCACGGGGAC GTGGTTTTCC TTTGAAAAAC ACGATGATAA
2101 TGGCCACAAC CATGGTGAGC AAGCAGATCC TGAAGAACAC CGGCCTGCAG
2151 GAGATCATGA GCTTCAAGGT GAACCTGGAG GGCGTGGTGA ACAACCACGT
2201 GTTCACCATG GAGGGCTGCG GCAAGGGCAA CATCCTGTTC GGCAACCAGC
2251 TGGTGCAGAT CCGCGTGACC AAGGGCGCCC CCCTGCCCTT CGCCTTCGAC
2301 ATCCTGAGCC CCGCCTTCCA GTACGGCAAC CGCACCTTCA CCAAGTACCC
2351 CGAGGACATC AGCGACTTCT TCATCCAGAG CTTCCCCGCC GGCTTCGTGT
2401 ACGAGCGCAC CCTGCGCTAC GAGGACGGCG GCCTGGTGGA GATCCGCAGC
2451 GACATCAACC TGATCGAGGA GATGTTCGTG TACCGCGTGG AGTACAAGGG
2501 CCGCAACTTC CCCAACGACG GCCCCGTGAT GAAGAAGACC ATCACCGGCC
2551 TGCAGCCCAG CTTCGAGGTG GTGTACATGA ACGACGGCGT GCTGGTGGGC
2601 CAGGTGATCC TGGTGTACCG CCTGAACAGC GGCAAGTTCT ACAGCTGCCA
2651 CATGCGCACC CTGATGAAGA GCAAGGGCGT GGTGAAGGAC TTCCCCGAGT
2701 ACCACTTCAT CCAGCACCGC CTGGAGAAGA CCTACGTGGA GGACGGCGGC
2751 TTCGTGGAGC AGCACGAGAC CGCCATCGCC CAGCTGACCA GCCTGGGCAA
2801 GCCCCTGGGC AGCCTGCACG AGTGGGTGTA ATAGGGTACC AGGTAAGTGT
2851 ACCCAATTCG CCCTATAGTG AGTCGTATTA GATCTACGGG TGGCATCCCT
2901 GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG
2951 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA
3001 GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG
3051 GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA
3101 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG
3151 GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG
3201 CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT
3251 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA
3301 CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC
3351 CCTTCCCTGT CCTTCTGATT TTGTAGGTAA CCACGTGCGG ACCGAGCGGC
3401 CGCAGGAACC CCTAGTGATG GAGTTGGCCA CTCCCTCTCT GCGCGCTCGC
3451 TCGCTCACTG AGGCCGGGCG ACCAAAGGTC GCCCGACGCC CGGGCTTTGC
3501 CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG CAGCTGCCTG CAGGGGCGCC
3551 TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA
3601 CGTCAAAGCA ACCATAGTAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG
3651 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC
3701 GCCCGCTCCT TTCGCTTTCT TCCCTTCCTTT CTCGCCACGT TCGCCGGCT
3751 TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT
3801 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTTGGGTG ATGGTTCACG
3851 TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT
3901 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC
3951 CCTATCTCGG GCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC
4001 CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA
4051 ACAAAATATT AACGTTTACA ATTTTATGGT GCACTCTCAG TACAATCTGC
4101 TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA CACCCGCTGA
4151 CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG
4201 TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG
4251 AAACGCGCGA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA
4301 TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA
4351 ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG
4401 TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA
4451 AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT
4501 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA
4551 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT
4601 GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT
4651 TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC
4701 CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA
4751 GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG
4801 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC
4851 ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC
4901 CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG
4951 AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG
5001 CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT
5051 TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG
5101 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT
5151 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT
5201 GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA
5251 GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC
5301 TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT
5351 TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA
5401 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC
5451 CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC
5501 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC
5551 CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG
5601 GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA
5651 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC
5701 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG
5751 TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG
5801 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA
5851 CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG
5901 CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG
5951 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT
6001 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA
6051 TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT
6101 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG Tagcattggt

关联产品
pHelper质粒DNA