人膀胱移行细胞癌UM-UC-3
Cat No.:JW309

种属：人
别称：UMUC-3; UM-UC3; UMUC3; UC-3; University of Michigan-Urothelial Carcinoma-3
组织来源：膀胱
疾病：移行细胞癌
传代比例/细胞消化：1:2传代 ，消化2-3分钟
完全培养基配置：MEM培养基 ；10%胎牛血清 ；1%双抗
简介：Derived from a urinary bladder transitional cell carcinoma from a human male. These cells are
tumourigenic in nude mice when 10E7 cells are inoculated subcutaneously. Hypertriploid. The modal chromosome number was 80, occurring in 42% of cells. Cells with 78 chromosomes also occurred at a high frequency. The rate of cells with higher ploidies was 2.5%. There were 30 or more marker
chromosomes in each cell. They included der(1)t (1;?) (p32;?), ?t(1p5p), i(3q), t(7q14q), ?t(2p3p) and others. The X and N3 had single copy per cell, and others were generally two or three copies per cell.
形态：上皮细胞样
生长特征：贴壁生长
倍增时间：每周 2 至 3 次
致瘤性：Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.
STR：Amelogenin: X CSF1PO: 10,11 D13S317: 8 D16S539: 8,9 D5S818: 12 D7S820: 8,9 THO1: 6,9 TPOX: 10
vWA: 17
培养条件：气相 ：空气 ，95% ；二氧化碳 ，5%。 温度 ：37摄氏度 ，培养箱湿度为70%-80%。
冻存条件：冻存液 ：90%FBS ，DMSO 10%,
或使用非程序冻存液 ：官网货号JW-H040
保藏机构：ATCC; CRL-1749
产品使用：仅限于科学研究 ，不可作为动物或人类疾病的治疗产品使用。

细胞接收处理流程 ：
1 ：观察有无破损漏液情况 ，如有请拍照及时联系客服。
2 ：酒精消毒培养瓶表面后显微镜下观察细胞状态 ，观察拍照后不用打开培养瓶盖 放入培养箱静止2-3小时稳定 细胞状态。
3 ：请按照细胞操作指南进行第一次传代冻存处理。
4 ：产品随货会附带细胞说明书、细胞培养操作指南、细胞鉴定、支原体检测报告。
5 ：若产品有异常或其他疑问 ，可随时联系客服 ；转至技术支持。