人神经母细胞瘤细胞SK-N-DZ
Cat No.:JW-J1192
种属:人
别称:SKNDZ
组织来源:大脑
疾病:神经母细胞瘤细胞
传代比例/细胞消化:1:2传代,
完全培养基配置:DMEM培养基 ;10%胎牛血清 ;1%双抗
简介:SK-N-DZ are neuroblasts that were isolated from the brain of a 2-year-old, White female patient with neuroblastoma.SK-N-DZ are neuroblasts that were isolated from the brain of a 2-year-old, White female patient with neuroblastoma. Expression of the N-myc gene product was reduced indifferentiated SK-N-DZ cells as compared with undifferentiated cells. Expression of the c-src geneproduct, pp60c-src was enhanced in differentiated SK-N-DZ cells as was tyrosine phosphorylation of cellular proteins. The cells exhibit moderate MDR1 expression. Retinoic acid induces differentiation in this line.
形态:成神经细胞样
生长特征:贴壁生长
STR:Amelogenin X CSF1PO 12 D3S1358 17 D5S818 12 D7S820 12,13 D8S1179 12,13 D13S317 8,11
D16S539 9,11 D18S51 15,17 D21S11 29,31 FGA 18,22 Penta D 9,14 Penta E 7,10 TH01 6,9 TPOX 8 vWA 16,18
倍增时间:每周 2 至 3 次
培养条件:气相:空气 ,95%;二氧化碳 ,5%。 温度:37摄氏度 ,培养箱湿度为70%-80%。
冻存条件:冻存液:90%FBS ,DMSO 10%,
或使用非程序冻存液:官网货号JW-H040
保藏机构:ATCC; CRL-2149
备注:该细胞贴壁松散 ,收货如有大块脱落或全部脱落的细胞团 ,为正常现象 ,请按照收货注意事项处理。
产品使用:仅限于科学研究 ,不可作为动物或人类疾病的治疗产品使用。
细胞接收处理流程:
1:观察有无破损漏液情况 ,如有请拍照及时联系客服。
2:酒精消毒培养瓶表面后显微镜下观察细胞状态 ,观察拍照后不用打开培养瓶盖 放入培养箱静止2-3小时稳定 细胞状态。
3:请按照细胞操作指南进行第一次传代冻存处理。
4:产品随货会附带细胞说明书、细胞培养操作指南、细胞鉴定、支原体检测报告。
5:若产品有异常或其他疑问 ,可随时联系客服;转至技术支持。