CAT#:JW-1-3795
常温运输及保存

pHelper质粒DNA

产品及特点
本产品为pHelper质粒DNA干粉。pHelper质粒DNA是含有pUC复制子的腺病毒包装载体，可用于同pAAV-MCS和pAAV-RC2质粒一起包装成腺相关病毒。本质粒携带Amp抗性基因，其图谱如下：

规格及成分
本产品使用塑料袋包装
成分                                   编号           规格        包装
pHelper质粒DNA干粉    1-3795           2 mg        0.5 mL本色管
使用手册                        1-3795sc        1 份        无

运输及保存：常温运输，低温保存，有效期两年
使用方法：使用前将本产品短暂离心后加入100μL自备超纯水或TE缓冲液溶解，得到20ng/μL的质粒DNA溶液，该溶液可直接用于腺病毒包装。也可以取少量，按常规方法进行细菌转化，制备更多的质粒DNA进行各种后续实验。
DNA序列
1 111 ggtacccaac tccatgctta acagtcccca ggtacagccc accctgcgtc
  151 gcaaccagga acagctctac agcttcctgg agcgccactc gccctacttc
  101 cgcagccaca gtgcgcagat taggagcgcc acttcttttt gtcacttgaa
  151 aaacatgtaa aaataatgta ctaggagaca ctttcaataa aggcaaatgt
  201 ttttatttgt acactctcgg gtgattattt accccccacc cttgccgtct
  251 gcgccgttta aaaatcaaag gggttctgcc gcgcatcgct atgcgccact
  301 ggcagggaca cgttgcgata ctggtgttta gtgctccact taaactcagg
  351 cacaaccatc cgcggcagct cggtgaagtt ttcactccac aggctgcgca
  401 ccatcaccaa cgcgtttagc aggtcgggcg ccgatatctt gaagtcgcag
  451 ttggggcctc cgccctgcgc gcgcgagttg cgatacacag ggttgcagca
  501 ctggaacact atcagcgccg ggtggtgcac gctggccagc acgctcttgt
  551 cggagatcag atccgcgtcc aggtcctccg cgttgctcag ggcgaacgga
  601 gtcaactttg gtagctgcct tcccaaaaag ggtgcatgcc caggctttga
  651 gttgcactcg caccgtagtg gcatcagaag gtgaccgtgc ccggtctggg
  701 cgttaggata cagcgcctgc atgaaagcct tgatctgctt aaaagccacc
  751 tgagcctttg cgccttcaga gaagaacatg ccgcaagact tgccggaaaa
  801 ctgattggcc ggacaggccg cgtcatgcac gcagcacctt gcgtcggtgt
  851 tggagatctg caccacattt cggccccacc ggttcttcac gatcttggcc
  901 ttgctagact gctccttcag cgcgcgctgc ccgttttcgc tcgtcacatc
  951 catttcaatc acgtgctcct tatttatcat aatgctcccg tgtagacact
 1001 taagctcgcc ttcgatctca gcgcagcggt gcagccacaa cgcgcagccc
 1051 gtgggctcgt ggtgcttgta ggttacctct gcaaacgact gcaggtacgc
 1101 ctgcaggaat cgccccatca tcgtcacaaa ggtcttgttg ctggtgaagg
 1151 tcagctgcaa cccgcggtgc tcctcgttta gccaggtctt gcatacggcc
 1201 gccagagctt ccacttggtc aggcagtagc ttgaagtttg cctttagatc
 1251 gttatccacg tggtacttgt ccatcaacgc gcgcgcagcc tccatgccct
 1301 tctcccacgc agacacgatc ggcaggctca gcgggtttat caccgtgctt
 1351 tcactttccg cttcactgga ctcttccttt tcctcttgcg tccgcatacc
 1401 ccgcgccact gggtcgtctt cattcagccg ccgcaccgtg cgcttacctc
 1451 ccttgccgtg cttgattagc accggtgggt tgctgaaacc caccatttgt
 1501 agcgccacat cttctctttc ttcctcgctg tccacgatca cctctgggga
 1551 tggcgggcgc tcgggcttgg gagaggggcg cttctttttc tttttggacg
 1601 caatggccaa atccgccgtc gaggtcgatg gccgcgggct gggtgtgcgc
 1651 ggcaccagcg catcttgtga cgagtcttct tcgtcctcgg actcgagacg
 1701 ccgcctcagc cgcttttttg ggggcgcgcg gggaggcggc ggcgacggcg
 1751 acggggacga cacgtcctcc atggttggtg gacgtcgcgc cgcaccgcgt
 1801 ccgcgctcgg gggtggtttc gcgctgctcc tcttcccgac tggccatttc
 1851 cttctcctat aggcagaaaa agatcatgga gtcagtcgag aaggaggaca
 1901 gcctaaccgc cccctttgag ttcgccacca ccgcctccac cgatgccgcc
 1951 aacgcgccta ccaccttccc cgtcgaggca cccccgcttg aggaggagga
 2001 agtgattatc gagcaggacc caggttttgt aagcgaagac gacgaggatc
 2051 gctcagtacc aacagaggat aaaaagcaag accaggacga cgcagaggca
 2101 aacgaggaac aagtcgggcg gggggaccaa aggcatggcg actacctaga
 2151 tgtgggagac gacgtgctgt tgaagcatct gcagcgccag tgcgccatta
 2201 tctgcgacgc gttgcaagag cgcagcgatg tgcccctcgc catagcggat
 2251 gtcagccttg cctacgaacg ccacctgttc tcaccgcgcg taccccccaa
 2301 acgccaagaa aacggcacat gcgagcccaa cccgcgcctc aacttctacc
 2351 ccgtatttgc cgtgccagag gtgcttgcca cctatcacat ctttttccaa
 2401 aactgcaaga tacccctatc ctgccgtgcc aaccgcagcc gagcggacaa
 2451 gcagctggcc ttgcggcagg gcgctgtcat acctgatatc gcctcgctcg
 2501 acgaagtgcc aaaaatcttt gagggtcttg gacgcgacga gaaacgcgcg
 2551 gcaaacgctc tgcaacaaga aaacagcgaa aatgaaagtc actgtggagt
 2601 gctggtggaa cttgagggtg acaacgcgcg cctagccgtg ctgaaacgca
 2651 gcatcgaggt cacccacttt gcctacccgg cacttaacct accccccaag
 2701 gttatgagca cagtcatgag cgagctgatc gtgcgccgtg cacgacccct
 2751 ggagagggat gcaaacttgc aagaacaaac cgaggagggc ctacccgcag
 2801 ttggcgatga gcagctggcg cgctggcttg agacgcgcga gcctgccgac
 2851 ttggaggagc gacgcaagct aatgatggcc gcagtgcttg ttaccgtgga
 2901 gcttgagtgc atgcagcggt tctttgctga cccggagatg cagcgcaagc
 2951 tagaggaaac gttgcactac acctttcgcc agggctacgt gcgccaggcc
 3001 tgcaaaattt ccaacgtgga gctctgcaac ctggtctcct accttggaat
 3051 tttgcacgaa aaccgcctcg ggcaaaacgt gcttcattcc acgctcaagg
 3101 gcgaggcgcg ccgcgactac gtccgcgact gcgtttactt atttctgtgc
 3151 tacacctggc aaacggccat gggcgtgtgg cagcaatgcc tggaggagcg
 3201 caacctaaag gagctgcaga agctgctaaa gcaaaacttg aaggacctat
 3251 ggacggcctt caacgagcgc tccgtggccg cgcacctggc ggacattatc
 3301 ttccccgaac gcctgcttaa aaccctgcaa cagggtctgc cagacttcac
 3351 cagtcaaagc atgttgcaaa actttaggaa ctttatccta gagcgttcag
 3401 gaattctgcc cgccacctgc tgtgcgcttc ctagcgactt tgtgcccatt
 3451 aagtaccgtg aatgccctcc gccgctttgg ggtcactgct accttctgca
 3501 gctagccaac taccttgcct accactccga catcatggaa gacgtgagcg
 3551 gtgacggcct actggagtgt cactgtcgct gcaacctatg caccccgcac
 3601 cgctccctgg tctgcaattc gcaactgctt agcgaaagtc aaattatcgg
 3651 tacctttgag ctgcagggtc cctcgcctga cgaaaagtcc gcggctccgg
 3701 ggttgaaact cactccgggg ctgtggacgt cggcttacct tcgcaaattt
 3751 gtacctgagg actaccacgc ccacgagatt aggttctacg aagaccaatc
 3801 ccgcccgcca aatgcggagc ttaccgcctg cgtcattacc cagggccaca
 3851 tccttggcca attgcaagcc atcaacaaag cccgccaaga gtttctgcta
 3901 cgaaagggac ggggggttta cctggacccc cagtccggcg aggagctcaa
 3951 cccaatcccc ccgccgccgc agccctatca gcagccgcgg gcccttgctt
 4001 cccaggatgg cacccaaaaa gaagctgcag ctgccgccgc cgccacccac
 4051 ggacgaggag gaatactggg acagtcaggc agaggaggtt ttggacgagg
 4101 aggaggagat gatggaagac tgggacagcc tagacgaagc ttccgaggcc
 4151 gaagaggtgt cagacgaaac accgtcaccc tcggtcgcat tcccctcgcc
 4201 ggcgccccag aaattggcaa ccgttcccag catcgctaca acctccgctc
 4251 ctcaggcgcc gccggcactg cctgttcgcc gacccaaccg tagatgggac
 4301 accactggaa ccagggccgg taagtctaag cagccgccgc cgttagccca
 4351 agagcaacaa cagcgccaag gctaccgctc gtggcgcggg cacaagaacg
 4401 ccatagttgc ttgcttgcaa gactgtgggg gcaacatctc cttcgcccgc
 4451 cgctttcttc tctaccatca cggcgtggcc ttcccccgta acatcctgca
 4501 ttactaccgt catctctaca gcccctactg caccggcggc agcggcagcg
 4551 gcagcaacag cagcggtcac acagaagcaa aggcgaccgg atagcaagac
 4601 tctgacaaag cccaagaaat ccacagcggc ggcagcagca ggaggaggag
 4651 cgctgcgtct ggcgcccaac gaacccgtat cgacccgcga gcttagaaat
 4701 aggatttttc ccactctgta tgctatattt caacaaagca ggggccaaga
 4751 acaagagctg aaaataaaaa acaggtctct gcgctccctc acccgcagct
 4801 gcctgtatca caaaagcgaa gatcagcttc ggcgcacgct ggaagacgcg
 4851 gaggctctct tcagcaaata ctgcgcgctg actcttaagg actagtttcg
 4901 cgccctttct caaatttaag cgcgaaaact acgtcatctc cagcggccac
 4951 acccggcgcc agcacctgtc gtcagcgcca ttatgagcaa ggaaattccc
 5001 acgccctaca tgtggagtta ccagccacaa atgggacttg cggctggagc
 5051 tgcccaagac tactcaaccc gaataaacta catgagcgcg ggaccccaca
 5101 tgatatcccg ggtcaacgga atccgcgccc accgaaaccg aattctcctc
 5151 gaacaggcgg ctattaccac cacacctcgt aataacctta atccccgtag
 5201 ttggcccgct gccctggtgt accaggaaag tcccgctccc accactgtgg
 5251 tacttcccag agacgcccag gccgaagttc agatgactaa ctcaggggcg
 5301 cagcttgcgg gcggctttcg tcacagggtg cggtcgcccg ggcgttttag
 5351 ggcggagtaa cttgcatgta ttgggaattg tagttttttt aaaatgggaa
 5401 gtgacgtatc gtgggaaaac ggaagtgaag atttgaggaa gttgtgggtt
 5451 ttttggcttt cgtttctggg cgtaggttcg cgtgcggttt tctgggtgtt
 5501 ttttgtggac tttaaccgtt acgtcatttt ttagtcctat atatactcgc
 5551 tctgtacttg gcccttttta cactgtgact gattgagctg gtgccgtgtc
 5601 gagtggtgtt ttttaatagg tttttttact ggtaaggctg actgttatgg
 5651 ctgccgctgt ggaagcgctg tatgttgttc tggagcggga gggtgctatt
 5701 ttgcctaggc aggagggttt ttcaggtgtt tatgtgtttt tctctcctat
 5751 taattttgtt atacctccta tgggggctgt aatgttgtct ctacgcctgc
 5801 gggtatgtat tcccccgggc tatttcggtc gctttttagc actgaccgat
 5851 gttaaccaac ctgatgtgtt taccgagtct tacattatga ctccggacat
 5901 gaccgaggaa ctgtcggtgg tgctttttaa tcacggtgac cagttttttt
 5951 acggtcacgc cggcatggcc gtagtccgtc ttatgcttat aagggttgtt
 6001 tttcctgttg taagacaggc ttctaatgtt taaatgtttt tttttttgtt
 6051 attttatttt gtgtttaatg caggaacccg cagacatgtt tgagagaaaa
 6101 atggtgtctt tttctgtggt ggttccggaa cttacctgcc tttatctgca
 6151 tgagcatgac tacgatgtgc ttgctttttt gcgcgaggct ttgcctgatt
 6201 ttttgagcag caccttgcat tttatatcgc cgcccatgca acaagcttac
 6251 ataggggcta cgctggttag catagctccg agtatgcgtg tcataatcag
 6301 tgtgggttct tttgtcatgg ttcctggcgg ggaagtggcc gcgctggtcc
 6351 gtgcagacct gcacgattat gttcagctgg ccctgcgaag ggacctacgg
 6401 gatcgcggta tttttgttaa tgttccgctt ttgaatctta tacaggtctg
 6451 tgaggaacct gaatttttgc aatcatgatt cgctgcttga ggctgaaggt
 6501 ggagggcgct ctggagcaga tttttacaat ggccggactt aatattcggg
 6551 atttgcttag agacatattg ataaggtggc gagatgaaaa ttatttgggc
 6601 atggttgaag gtgctggaat gtttatagag gagattcacc ctgaagggtt
 6651 tagcctttac gtccacttgg acgtgagggc agtttgcctt ttggaagcca
 6701 ttgtgcaaca tcttacaaat gccattatct gttctttggc tgtagagttt
 6751 gaccacgcca ccggagggga gcgcgttcac ttaatagatc ttcattttga
 6801 ggttttggat aatcttttgg aataaaaaaa aaaaaacatg gttcttccag
 6851 ctcttcccgc tcctcccgtg tgtgactcgc agaacgaatg tgtaggttgg
 6901 ctgggtgtgg cttattctgc ggtggtggat gttatcaggg cagcggcgca
 6951 tgaaggagtt tacatagaac ccgaagccag ggggcgcctg gatgctttga
 7001 gagagtggat atactacaac tactacacag agcgagctaa gcgacgagac
 7051 cggagacgca gatctgtttg tcacgcccgc acctggtttt gcttcaggaa
 7101 atatgactac gtccggcgtt ccatttggca tgacactacg accaacacga
 7151 tctcggttgt ctcggcgcac tccgtacagt agggatcgcc tacctccttt
 7201 tgagacagag acccgcgcta ccatactgga ggatcatccg ctgctgcccg
 7251 aatgtaacac tttgacaatg cacaacgtga gttacgtgcg aggtcttccc
 7301 tgcagtgtgg gatttacgct gattcaggaa tgggttgttc cctgggatat
 7351 ggttctgacg cgggaggagc ttgtaatcct gaggaagtgt atgcacgtgt
 7401 gcctgtgttg tgccaacatt gatatcatga cgagcatgat gatccatggt
 7451 tacgagtcct gggctctcca ctgtcattgt tccagtcccg gttccctgca
 7501 gtgcatagcc ggcgggcagg ttttggccag ctggtttagg atggtggtgg
 7551 atggcgccat gtttaatcag aggtttatat ggtaccggga ggtggtgaat
 7601 tacaacatgc caaaagaggt aatgtttatg tccagcgtgt ttatgagggg
 7651 tcgccactta atctacctgc gcttgtggta tgatggccac gtgggttctg
 7701 tggtccccgc catgagcttt ggatacagcg ccttgcactg tgggattttg
 7751 aacaatattg tggtgctgtg ctgcagttac tgtgctgatt taagtgagat
 7801 cagggtgcgc tgctgtgccc ggaggacaag gcgtctcatg ctgcgggcgg
 7851 tgcgaatcat cgctgaggag accactgcca tgttgtattc ctgcaggacg
 7901 gagcggcggc ggcagcagtt tattcgcgcg ctgctgcagc accaccgccc
 7951 tatcctgatg cacgattatg actctacccc catgtaggcg tggacttccc
 8001 cttcgccgcc cgttgagcaa ccgcaagttg gacagcagcc tgtggctcag
 8051 cagctggaca gcgacatgaa cttaagcgag ctgcccgggg agtttattaa
 8101 tatcactgat gagcgtttgg ctcgacagga aaccgtgtgg aatataacac
 8151 ctaagaatat gtctgttacc catgatatga tgctttttaa ggccagccgg
 8201 ggagaaagga ctgtgtactc tgtgtgttgg gagggaggtg gcaggttgaa
 8251 tactagggtt ctgtgagttt gattaaggta cggtgatcaa tataagctat
 8301 gtggtggtgg ggctatacta ctgaatgaaa aatgacttga aattttctgc
 8351 aattgaaaaa taaacacgtt gaaacataac atgcaacagg ttcacgattc
 8401 tttattcctg ggcaatgtag gagaaggtgt aagagttggt agcaaaagtt
 8451 tcagtggtgt attttccact ttcccaggac catgtaaaag acatagagta
 8501 agtgcttacc tcgctagttt ctgtggattc actagaatcg atgtaggatg
 8551 ttgcccctcc tgacgcggta ggagaagggg agggtgccct gcatgtctgc
 8601 cgctgctctt gctcttgccg ctgctgagga ggggggcgca tctgccgcag
 8651 caccggatgc atctgggaaa agcaaaaaag gggctcgtcc ctgtttccgg
 8701 aggaatttgc aagcggggtc ttgcatgacg gggaggcaaa cccccgttcg
 8751 ccgcagtccg gccggcccga gactcgaacc gggggtcctg cgactcaacc
 8801 cttggaaaat aaccctccgg ctacagggag cgagccactt aatgctttcg
 8851 ctttccagcc taaccgctta cgccgcgcgc ggccagtggc caaaaaagct
 8901 agcgcagcag ccgccgcgcc tggaaggaag ccaaaaggag cgctcccccg
 8951 ttgtctgacg tcgcacacct gggttcgaca cgcgggcggt aaccgcatgg
 9001 atcacggcgg acggccggat ccggggttcg aaccccggtc gtccgccatg
 9051 atacccttgc gaatttatcc accagaccac ggaagagtgc ccgcttacag
 9101 gctctccttt tgcacggtct agagcgtcaa cgactgcgca cgcctcaccg
 9151 gccagagcgt cccgaccatg gagcactttt tgccgctgcg caacatctgg
 9201 aaccgcgtcc gcgactttcc gcgcgcctcc accaccgccg ccggcatcac
 9251 ctggatgtcc aggtacatct acggattacg tcgacgttta aaccatatga
 9301 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
 9351 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
 9401 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
 9451 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
 9501 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
 9551 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
 9601 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
 9651 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
 9701 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
 9751 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
 9801 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
 9851 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
 9901 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
 9951 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
10001 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
10051 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
10101 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
10151 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
10201 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
10251 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
10301 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
10351 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
10401 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
10451 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
10501 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
10551 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
10601 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
10651 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
10701 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
10751 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
10801 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
10851 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
10901 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
10951 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
11001 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
11051 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
11101 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
11151 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
11201 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
11251 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
11301 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
11351 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
11401 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
11451 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
11501 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
11551 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
11601 gcgcttaatg cgccgctaca gggcgcgatg gatcccATtt cggggcagtc
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