CAT#:JW-1-3793
常温运输及保存

pAAV-RC2质粒DNA

产品及特点
本产品为pAAV-RC2质粒DNA干粉，是含有pUC复制子的腺病毒包装载体。pAAV-RC2质粒DNA含有Cap和Rep基因，可与pAAV-MCS和pHelper质粒一起包装成腺相关病毒。本质粒携带Amp抗性基因，其图谱如下：

规格及成分
本产品使用塑料袋包装    
成分                                      编号            规格        包装
pAAV-RC2质粒DNA干粉    1-3793           2 mg        0.5 mL本色管
使用手册                             1-3793sc        1 份        无

运输及保存：常温运输，低温保存，有效期两年
使用方法：使用前将本产品短暂离心后加入100μL自备超纯水或TE缓冲液溶解，得到20ng/μL的质粒DNA溶液，该溶液可直接用于腺病毒包装。也可以取少量，按常规方法进行细菌转化，制备更多的质粒DNA进行各种后续实验。
DNA序列
11 1 gcgcgccgat atcgttaacg ccccgcgccg gccgctctag aactagtgga
 151 tcccccggaa gatcagaagt tcctattccg aagttcctat tctctagaaa
 101 gtataggaac ttctgatctg cgcagccgcc atgccggggt tttacgagat
 151 tgtgattaag gtccccagcg accttgacga gcatctgccc ggcatttctg
 201 acagctttgt gaactgggtg gccgagaagg aatgggagtt gccgccagat
 251 tctgacatgg atctgaatct gattgagcag gcacccctga ccgtggccga
 301 gaagctgcag cgcgactttc tgacggaatg gcgccgtgtg agtaaggccc
 351 cggaggccct tttctttgtg caatttgaga agggagagag ctacttccac
 401 atgcacgtgc tcgtggaaac caccggggtg aaatccatgg ttttgggacg
 451 tttcctgagt cagattcgcg aaaaactgat tcagagaatt taccgcggga
 501 tcgagccgac tttgccaaac tggttcgcgg tcacaaagac cagaaatggc
 551 gccggaggcg ggaacaaggt ggtggatgag tgctacatcc ccaattactt
 601 gctccccaaa acccagcctg agctccagtg ggcgtggact aatatggaac
 651 agtatttaag cgcctgtttg aatctcacgg agcgtaaacg gttggtggcg
 701 cagcatctga cgcacgtgtc gcagacgcag gagcagaaca aagagaatca
 751 gaatcccaat tctgatgcgc cggtgatcag atcaaaaact tcagccaggt
 801 acatggagct ggtcgggtgg ctcgtggaca aggggattac ctcggagaag
 851 cagtggatcc aggaggacca ggcctcatac atctccttca atgcggcctc
 901 caactcgcgg tcccaaatca aggctgcctt ggacaatgcg ggaaagatta
 951 tgagcctgac taaaaccgcc cccgactacc tggtgggcca gcagcccgtg
1001 gaggacattt ccagcaatcg gatttataaa attttggaac taaacgggta
1051 cgatccccaa tatgcggctt ccgtctttct gggatgggcc acgaaaaagt
1101 tcggcaagag gaacaccatc tggctgtttg ggcctgcaac taccgggaag
1151 accaacatcg cggaggccat agcccacact gtgcccttct acgggtgcgt
1201 aaactggacc aatgagaact ttcccttcaa cgactgtgtc gacaagatgg
1251 tgatctggtg ggaggagggg aagatgaccg ccaaggtcgt ggagtcggcc
1301 aaagccattc tcggaggaag caaggtgcgc gtggaccaga aatgcaagtc
1351 ctcggcccag atagacccga ctcccgtgat cgtcacctcc aacaccaaca
1401 tgtgcgccgt gattgacggg aactcaacga ccttcgaaca ccagcagccg
1451 ttgcaagacc ggatgttcaa atttgaactc acccgccgtc tggatcatga
1501 ctttgggaag gtcaccaagc aggaagtcaa agactttttc cggtgggcaa
1551 aggatcacgt ggttgaggtg gagcatgaat tctacgtcaa aaagggtgga
1601 gccaagaaaa gacccgcccc cagtgacgca gatataagtg agcccaaacg
1651 ggtgcgcgag tcagttgcgc agccatcgac gtcagacgcg gaagcttcga
1701 tcaactacgc agacaggtac caaaacaaat gttctcgtca cgtgggcatg
1751 aatctgatgc tgtttccctg cagacaatgc gagagaatga atcagaattc
1801 aaatatctgc ttcactcacg gacagaaaga ctgtttagag tgctttcccg
1851 tgtcagaatc tcaacccgtt tctgtcgtca aaaaggcgta tcagaaactg
1901 tgctacattc atcatatcat gggaaaggtg ccagacgctt gcactgcctg
1951 cgatctggtc aatgtggatt tggatgactg catctttgaa caataaatga
2001 tttaaatcag gtatggctgc cgatggttat cttccagatt ggctcgagga
2051 cactctctct gaaggaataa gacagtggtg gaagctcaaa cctggcccac
2101 caccaccaaa gcccgcagag cggcataagg acgacagcag gggtcttgtg
2151 cttcctgggt acaagtacct cggacccttc aacggactcg acaagggaga
2201 gccggtcaac gaggcagacg ccgcggccct cgagcacgac aaagcctacg
2251 accggcagct cgacagcgga gacaacccgt acctcaagta caaccacgcc
2301 gacgcggagt ttcaggagcg ccttaaagaa gatacgtctt ttgggggcaa
2351 cctcggacga gcagtcttcc aggcgaaaaa gagggttctt gaacctctgg
2401 gcctggttga ggaacctgtt aagacggctc cgggaaaaaa gaggccggta
2451 gagcactctc ctgtggagcc agactcctcc tcgggaaccg gaaaggcggg
2501 ccagcagcct gcaagaaaaa gattgaattt tggtcagact ggagacgcag
2551 actcagtacc tgacccccag cctctcggac agccaccagc agccccctct
2601 ggtctgggaa ctaatacgat ggctacaggc agtggcgcac caatggcaga
2651 caataacgag ggcgccgacg gagtgggtaa ttcctcggga aattggcatt
2701 gcgattccac atggatgggc gacagagtca tcaccaccag cacccgaacc
2751 tgggccctgc ccacctacaa caaccacctc tacaaacaaa tttccagcca
2801 atcaggagcc tcgaacgaca atcactactt tggctacagc accccttggg
2851 ggtattttga cttcaacaga ttccactgcc acttttcacc acgtgactgg
2901 caaagactca tcaacaacaa ctggggattc cgacccaaga gactcaactt
2951 caagctcttt aacattcaag tcaaagaggt cacgcagaat gacggtacga
3001 cgacgattgc caataacctt accagcacgg ttcaggtgtt tactgactcg
3051 gagtaccagc tcccgtacgt cctcggctcg gcgcatcaag gatgcctccc
3101 gccgttccca gcagacgtct tcatggtgcc acagtatgga tacctcaccc
3151 tgaacaacgg gagtcaggca gtaggacgct cttcatttta ctgcctggag
3201 tactttcctt ctcagatgct gcgtaccgga aacaacttta ccttcagcta
3251 cacttttgag gacgttcctt tccacagcag ctacgctcac agccagagtc
3301 tggaccgtct catgaatcct ctcatcgacc agtacctgta ttacttgagc
3351 agaacaaaca ctccaagtgg aaccaccacg cagtcaaggc ttcagttttc
3401 tcaggccgga gcgagtgaca ttcgggacca gtctaggaac tggcttcctg
3451 gaccctgtta ccgccagcag cgagtatcaa agacatctgc ggataacaac
3501 aacagtgaat actcgtggac tggagctacc aagtaccacc tcaatggcag
3551 agactctctg gtgaatccgg gcccggccat ggcaagccac aaggacgatg
3601 aagaaaagtt ttttcctcag agcggggttc tcatctttgg gaagcaaggc
3651 tcagagaaaa caaatgtgga cattgaaaag gtcatgatta cagacgaaga
3701 ggaaatcagg acaaccaatc ccgtggctac ggagcagtat ggttctgtat
3751 ctaccaacct ccagagaggc aacagacaag cagctaccgc agatgtcaac
3801 acacaaggcg ttcttccagg catggtctgg caggacagag atgtgtacct
3851 tcaggggccc atctgggcaa agattccaca cacggacgga cattttcacc
3901 cctctcccct catgggtgga ttcggactta aacaccctcc tccacagatt
3951 ctcatcaaga acaccccggt acctgcgaat ccttcgacca ccttcagtgc
4001 ggcaaagttt gcttccttca tcacacagta ctccacggga caggtcagcg
4051 tggagatcga gtgggagctg cagaaggaaa acagcaaacg ctggaatccc
4101 gaaattcagt acacttccaa ctacaacaag tctgttaatg tggactttac
4151 tgtggacact aatggcgtgt attcagagcc tcgccccatt ggcaccagat
4201 acctgactcg taatctgtaa ttgcttgtta atcaataaac cgtttaattc
4251 gtttcagttg aactttggtc tctgcgtatt tctttcttat ctagtttcca
4301 tggctacgta gataagtagc atggcgggtt aatcattaac tacagcccgg
4351 gcgtttaaac agcgggcgga ggggtggagt cgtgacgtga attacgtcat
4401 agggttaggg aggtcctgta ttagaggtca cgtgagtgtt ttgcgacatt
4451 ttgcgacacc atgtggtctc gctggggggg ggggcccgag tgagcacgca
4501 gggtctccat tttgaagcgg gaggtttgaa cgagcgctgg cgcgctcact
4551 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
4601 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
4651 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
4701 atggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
4751 ttaaatcagc tcattttttt aaccaatagg ccgaaatcgg caaaatccct
4801 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
4851 gaacaagagt ccactattaa gaacgtggac tccaacgtca aagggcgaaa
4901 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
4951 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
5001 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
5051 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
5101 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
5151 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
5201 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
5251 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
5301 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
5351 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
5401 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
5451 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
5501 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
5551 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
5601 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
5651 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
5701 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
5751 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
5801 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
5851 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
5901 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
5951 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
6001 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
6051 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
6101 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
6151 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
6201 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
6251 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
6301 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
6351 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
6401 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
6451 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
6501 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
6551 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
6601 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
6651 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
6701 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
6751 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
6801 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
6851 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
6901 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
6951 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
7001 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
7051 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
7101 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
7151 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
7201 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
7251 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
7301 aaacagctat gaccatgatt acgccaactt cggggcagtc ctcggcccaa
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