CAT#:JW-1-4540y 常温运输，常温保存

pLKO.1-TRC cloning vector 质粒 DNA
pLKO.1-TRC cloning vector Plasmid DNA
    使用手册 V1.0

产品及特点    
pLKO.1-TRC cloning vector 质粒 DNA ，常用于基因工程和分子生物学研究。 其图谱如下：

规格及成分    
本产品使用塑料袋包装
成分                                                                        编号    规格    包装
pLKO.1-TRC cloning vector 质粒 DNA 干粉        1-4540y    2 μg    0.5 mL 本色管
使用手册                                                              1-4540ysc    1份    无
运输及保存：低温运输，-20℃保存，有效期两年。
使用方法    
使用前将本产品短暂离心后加入 100  μL  自备超纯水或 TE 缓冲液溶解，得到 20 ng/ μL 的质粒 DNA 溶液，该溶液可直接用于酶切克隆。也可以取少量，按常规方法进 行细菌转化，制备更多的质粒 DNA 进行各种后续实验。

DNA 序列    
1 CACCGTCGAT CCGGACCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC TCTTCCTCAC
61 GCGCGTCGGG CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG CGGTGGCGGT
121 CTGGACCACG CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT
181 GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC
241 GCACCGGCCC AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC CCGACCACCA
301 GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG
361 GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG AGCGGCTCGG
421 CTTCACCGTC ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT GCATGACCCG
481 CAAGCCCGGT GCCTGACGCC CGCCCCACGA CCCGCAGCGC CCGACCGAAA GGAGCGCACG
541 ACCCCATGCA TCGGTACCTT TAAGACCAAT GACTTACAAG GCAGCTGTAG ATCTTAGCCA
601 CTTTTTAAAA GAAAAGGGGG GACTGGAAGG GCTAATTCAC TCCCAACGAA GACAAGATCT
661 GCTTTTTGCT TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG
721 CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT
781 GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT
841 GTGGAAAATC TCTAGCAGTA GTAGTTCATG TCATCTTATT ATTCAGTATT TATAACTTGC
901 AAAGAAATGA ATATCAGAGA GTGAGAGGAA CTTGTTTATT GCAGCTTATA ATGGTTACAA
961 ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG
1021 TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG CTCTAGCTAT CCCGCCCCTA
1081 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA
1141 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG
1201 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGG ACGTACCCAA TTCGCCCTAT AGTGAGTCGT
1261 ATTACGCGCG CTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA
1321 CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG
1381 CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG GACGCGCCCT
1441 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG
1501 CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG
1561 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC
1621 GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT
1681 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT
1741 TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT
1801 TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT
1861 TTAACAAAAT ATTAACGCTT ACAATTTAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC
1921 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC
1981 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT
2041 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT
2101 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA
2161 TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG
2221 CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA
2281 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA
2341 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG
2401 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC
2461 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA
2521 TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT
2581 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG
2641 GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT
2701 TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG
2761 GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT
2821 GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT
2881 GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA
2941 AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT
3001 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT
3061 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG
3121 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA
3181 GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT
3241 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA
3301 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC
3361 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT
3421 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA
3481 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG
3541 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT
3601 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT
3661 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA
3721 TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC
3781 GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC
3841 TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA
3901 AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC
3961 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA
4021 CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCGCGCA ATTAACCCTC ACTAAAGGGA
4081 ACAAAAGCTG GAGCTGCAAG CTTAATGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC
4141 ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA
4201 TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA
4261 TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT
4321 CGATACATAA ACGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA
4381 CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT
4441 GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG
4501 AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA CCAGAGGAGC
4561 TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG GGGCGGCGAC
4621 TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT GGGTGCGAGA
4681 GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG TTAAGGCCAG
4741 GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG CTAGAACGAT
4801 TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA CTGGGACAGC
4861 TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT ACAGTAGCAA
4921 CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT TTAGACAAGA
4981 TAGAGGAAGA GCAAAACAAA AGTAAGACCA CCGCACAGCA AGCGGCCGCT GATCTTCAGA
5041 CCTGGAGGAG GAGATATGAG GGACAATTGG AGAAGTGAAT TATATAAATA TAAAGTAGTA
5101 AAAATTGAAC CATTAGGAGT AGCACCCACC AAGGCAAAGA GAAGAGTGGT GCAGAGAGAA
5161 AAAAGAGCAG TGGGAATAGG AGCTTTGTTC CTTGGGTTCT TGGGAGCAGC AGGAAGCACT
5221 ATGGGCGCAG CGTCAATGAC GCTGACGGTA CAGGCCAGAC AATTATTGTC TGGTATAGTG
5281 CAGCAGCAGA ACAATTTGCT GAGGGCTATT GAGGCGCAAC AGCATCTGTT GCAACTCACA
5341 GTCTGGGGCA TCAAGCAGCT CCAGGCAAGA ATCCTGGCTG TGGAAAGATA CCTAAAGGAT
5401 CAACAGCTCC TGGGGATTTG GGGTTGCTCT GGAAAACTCA TTTGCACCAC TGCTGTGCCT
5461 TGGAATGCTA GTTGGAGTAA TAAATCTCTG GAACAGATTT GGAATCACAC GACCTGGATG
5521 GAGTGGGACA GAGAAATTAA CAATTACACA AGCTTAATAC ACTCCTTAAT TGAAGAATCG
5581 CAAAACCAGC AAGAAAAGAA TGAACAAGAA TTATTGGAAT TAGATAAATG GGCAAGTTTG
5641 TGGAATTGGT TTAACATAAC AAATTGGCTG TGGTATATAA AATTATTCAT AATGATAGTA
5701 GGAGGCTTGG TAGGTTTAAG AATAGTTTTT GCTGTACTTT CTATAGTGAA TAGAGTTAGG
5761 CAGGGATATT CACCATTATC GTTTCAGACC CACCTCCCAA CCCCGAGGGG ACCCGACAGG
5821 CCCGAAGGAA TAGAAGAAGA AGGTGGAGAG AGAGACAGAG ACAGATCCAT TCGATTAGTG
5881 AACGGATCTC GACGGTATCG ATCACGAGAC TAGCCTCGAG CGGCCGCCCC CTTCACCGAG
5941 GGCCTATTTC CCATGATTCC TTCATATTTG CATATACGAT ACAAGGCTGT TAGAGAGATA
6001 ATTGGAATTA ATTTGACTGT AAACACAAAG ATATTAGTAC AAAATACGTG ACGTAGAAAG
6061 TAATAATTTC TTGGGTAGTT TGCAGTTTTA AAATTATGTT TTAAAATGGA CTATCATATG
6121 CTTACCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA
6181 AACACCGGTG TGTTGTAAAT GAGCACACAA AATACACATG CTAAAATATT ATATTCTATG
6241 ACCTTTATAA AATCAACCAA AATCTTCTTT TTAATAACTT TAGTATCAAT AATTAGAATT
6301 TTTATGTTCC TTTTTGCAAA CTTTTAATAA AAATGAGCAA AATAAAAAAA CGCTAGTTTT
6361 AGTAACTCGC GTTGTTTTCT TCACCTTTAA TAATAGCTAC TCCACCACTT GTTCCTAAGC
6421 GGTCAGCTCC TGCTTCAATC ATTTTTTGAG CATCTTCAAA TGTTCTAACT CCACCAGCTG
6481 CTTTAACTAA AGCATTGTCT TTAACAACTG ACTTCATTAG TTTAACATCT TCAAATGTTG
6541 CACCTGATTT TGAAAATCCT GTTGATGTTT TAACAAATTC TAATCCAGCT TCAACAGCTA
6601 TTTCACAAGC TTTCATGATT TCTTCTTTTG TTAATAAACA ATTTTCCATA ATACATTTAA
6661 CAACATGTGA TCCAGCTGCT TTTTTTACAG CTTTCATGTC TTCTAAAACT AATTCATAAT
6721 TTTTGTCTTT TAATGCACCA ATATTTAATA CCATATCAAT TTCTGTTGCA CCATCTTTAA
6781 TTGCTTCAGA AACTTCGAAT GCTTTTGTAG CTGTTGTGCA TGCACCTAGA GGAAAACCTA
6841 CAACATTTGT TATTCCTACA TTTGTGCCTT TTAATAATTC TTTACAATAG CTTGTTCAAT
6901 ATGAATTAAC ACAAACTGTT GCAAAATCAA ATTCAATTGC TTCATCACAT AATTGTTTAA
6961 TTTCAGCTTT CGTAGCATCT TGTTTTAATA ATGTGTGATC TATATATTTG TTTAGTTTCA
7021 TTTTTTCTCC TATATATTCA TTTTTAATTT TAATTCTTTA ATAATTTCGT CTACTTTAAC
7081 TTTAGCGTTT TGAACAGATT CACCAACACC TATAAAATAA ATTTTTAGTT TAGGTTCAGT
7141 TCCACTTGGG CGAACAGCAA ATCATGACTT ATCTTCTAAA TAAAATTTTA GTAAGTCTTG
7201 TCCTGGCATA TTATACATTC CATCGATGTA GTCTTCAACA TTAACAACTT TAAGTCCAGC
7261 AATTTGAGTT AAGGGTGTTG CTCTCAATGA TTTCATTAAT GGTTCAATTT TTAATTTCTT
7321 TTCTTCTGGT TTAAAATTCA AGTTTAAAGT GAAAGTGTAA TATGCACCCA TTTCTTTAAA
7381 TAAATCTTCT AAATAGTCTA CTAATGTTTT ATTTTGTTTT TTATAAAATC AAGCAGCCTC
7441 TGCTATTAAT ATAGAAGCTT GTATTCCATC TTTATCTCTA GCTGAGTCAT CAATTACATA
7501 TCCATAACTT TCTTCATAAG CAAAAACAAA ATTTAATCCG TTATCTTCTT CTTTAGCAAT
7561 TTCTCTACCC ATTCATTTAA ATCCAGTTAA AGTTTTTACA ATATTAACTC CATATTTTTC
7621 ATGAGCGATT CTATCACCCA AATCACTTGT TACAAAACTT GAATATAGAG CCGGATTTTT
7681 TGGAATGCTA TTTAAGCGTT TTAGATTTGA TAATTTTCAA TCAATTAAAA TTGGTCCTGT
7741 TTGATTTCCA TCTAATCTTA CAAAATGACC ATCATGTTTT ATTGCCATTC CAAATCTGTC
7801 AGCATCTGGG TCATTCATAA TAATAATATC TGCATCATGT TTAATACCAT ATTCAAGCGG
7861 TATTTTTCAT GCAGGATCAA ATTCTGGATT TGGATTTACA ACATTTTTAA ATGTTTCATC
7921 TTCAAATGCA TGCTCTTCAA CCTCAATAAC GTTATATCCT GATTCACGTA ATATTTTTGG
7981 GGTAAATTTA GTTCCTGTTC CATTAACTGC GCTAAAAATA ATTTTTAAAT CTTTTTTAGC
8041 TTCTTGCTCT TTTTTGTAGA ATTCTCGACC TCGAGACAAA TGGCAGTATT CATCCACAAT
8101 TTTAAAAGAA AAGGGGGGAT TGGGGGGTAC AGTGCAGGGG AAAGAATAGT AGACATAATA
8161 GCAACAGACA TACAAACTAA AGAATTACAA AAACAAATTA CAAAAATTCA AAATTTTCGG
8221 GTTTATTACA GGGACAGCAG AGATCCACTT TGGCCGCGGC TCGAGGGGGT TGGGGTTGCG
8281 CCTTTTCCAA GGCAGCCCTG GGTTTGCGCA GGGACGCGGC TGCTCTGGGC GTGGTTCCGG
8341 GAAACGCAGC GGCGCCGACC CTGGGTCTCG CACATTCTTC ACGTCCGTTC GCAGCGTCAC
8401 CCGGATCTTC GCCGCTACCC TTGTGGGCCC CCCGGCGACG CTTCCTGCTC CGCCCCTAAG
8461 TCGGGAAGGT TCCTTGCGGT TCGCGGCGTG CCGGACGTGA CAAACGGAAG CCGCACGTCT
8521 CACTAGTACC CTCGCAGACG GACAGCGCCA GGGAGCAATG GCAGCGCGCC GACCGCGATG
8581 GGCTGTGGCC AATAGCGGCT GCTCAGCAGG GCGCGCCGAG AGCAGCGGCC GGGAAGGGGC
8641 GGTGCGGGAG GCGGGGTGTG GGGCGGTAGT GTGGGCCCTG TTCCTGCCCG CGCGGTGTTC
8701 CGCATTCTGC AAGCCTCCGG AGCGCACGTC GGCAGTCGGC TCCCTCGTTG ACCGAATCAC
8761 CGACCTCTCT CCCCAGGGGG ATCCACCGGA GCTTACCATG ACCGAGTACA AGCCCACGGT
8821 GCGCCTCGCC ACCCGCGACG ACGTCCCCAG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC
8881 CGACTACCCC GCCACGCGCC A

    
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